embryogenic callus production in four grapevine (vitisvinifera l.) cultivars using anther and whole flower culture

نویسندگان

مریم کریمی علویجه

پردیس کشاورزی و منابع طبیعی دانشگاه تهران، دانشجوی سابق کارشناسی ارشد علی عبادی

استاد پردیس کشاورزی و منابع طبیعی دانشگاه تهران منصور امیدی

استاد پردیس کشاورزی و منابع طبیعی دانشگاه تهران

چکیده

embryogenic callus initiation from anther and whole flower explants in four grapevine cultivars including flame seedless, shahroodi, perlette and red sultanina cultured on piv and harst nutrient media was studied. results showed that whole flower explants of ‘flame seedless’ cultured on piv medium had the most potential for callus production. later callus were transferred onto ges1ca medium for embryogenesis. in the latter medium, ‘shahroodi’ expressed the highest rate of embryogenic callus production as compared with the other three cultivars. meanwhile, only the anther explants showed the potential for producing embryogenic callus. keywords: grape, media, explant, callus production, and embryogenic callus

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Characterization of Four Famous Red Grapevine Cultivars

The grape berries due to containing organic acids, sugars, aromatic compounds, phenolic compounds (including anthocyanins, flavanols, flavonols, stilbenes (resveratrol)), tannins, and oil in the pulp, skin, and seed have numerous health benefits for human health. In this study, we investigated genetic and phytochemical characteristics of four famous grapevine cultivars (Shiraz, Sirch, Panje Aro...

متن کامل

Biochemical studies of embryogenic and non-embryogenic callus of Cardiospermum halicacabum L.

Some physiological and biochemical changes were measured between embryogenic and non-embryogenic callus obtained from Cardiospermum halicacabum. Combination of auxin with cytokinin was more favourable for high amount of callus formation. 2,4-D played a key role in triggering somatic embryo formation. Embryogenic callus had more total carbohydrate and starch contents, total free amino acids, nuc...

متن کامل

Sequence Polymorphisms and Structural Variations among Four Grapevine (Vitis vinifera L.) Cultivars Representing Sardinian Agriculture

The genetic diversity among grapevine (Vitis vinifera L.) cultivars that underlies differences in agronomic performance and wine quality reflects the accumulation of single nucleotide polymorphisms (SNPs) and small indels as well as larger genomic variations. A combination of high throughput sequencing and mapping against the grapevine reference genome allows the creation of comprehensive seque...

متن کامل

Somaclonal variation on in vitro callus culture potato cultivars

Hortic. bras., v. 22, n. 2, abr.-jun. 2004 C potato (Solanum tuberosum L.) is one of the most important vegetable crop of Brazil and the world’s fourth most important crop (Solomon-Blackbourn & Barker, 2001). In 2002, Brazil produced 3,126,411 t of potato with an average yield of 19,4 kg/ ha (IBGE, 2002). Potato is a tetraploid species and the use of its botanical seeds in commercial cultivatio...

متن کامل

Cryopreservation of embryogenic cell suspensions of the Spanish grapevine cultivars ‘Albariño’ and ‘Tempranillo’

Embryogenic cell suspensions of two elite Spanish grapevine cultivars (‘Albariño’ and ‘Tempranillo’) were successfully cryopreserved by encapsulation-dehydration. The method implied the encapsulation of cells in alginate beads, subsequent culture in liquid medium with 1 M sucrose for 4 days, and desiccation for 2-4 h in the flow of a laminar-flow bench, before immersing in liquid nitrogen. With...

متن کامل

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023